ABSTRACT
OBJETIVO: Comparar o desempenho de duas técnicas de genotipagem de papilomavírus humano (HPV), Linear Array e PapilloCheck, em mulheres com lesão intra-epitelial de alto grau (LIAG). MÉTODOS: Foram selecionadas 88 mulheres com diagnóstico citopatológico de LIAG em 2 centros de referência em patologia cervical em Salvador, Bahia, no período de julho de 2006 a janeiro de 2009. Após o diagnóstico citopatológico de LIAG, foram realizadas a coleta de células do colo uterino para a genotipagem do HPV e a biópsia sob visão colposcópica para análise histopatológica do fragmento retirado. Posteriormente à confirmação de NIC2+ pelo exame histopatológico, foi realizada a genotipagem do HPV em 41 mulheres pelas técnicas Linear Array e PapilloCheck. RESULTADOS: Os dois testes apresentaram taxa de concordância geral para detecção do vírus HPV de 97,2% (35/36). Das 36 amostras válidas, 35 (97,2%) foram consideradas positivas em ambos os testes e apenas uma amostra (2,8%) foi considerada discordante. Os genótipos do HPV mais prevalentes detectados através da técnica do Linear Array foram: HPV 16, HPV 56, HPV 35, HPV 45 e HPV 70; e pela técnica PapilloCheck foram: HPV 16, HPV 56, HPV 11, HPV 35 e HPV 42. Foi observado índice semelhante de infecção por múltiplos tipos do HPV nos dois testes analisados (72,5% no Linear Array e 75,0% no PapilloCheck). CONCLUSÕES: Os testes de genotipagem Linear Array e PapilloCheck apresentaram um desempenho equivalente na detecção dos tipos de HPV oncogênicos em mulheres com LIAG, tendo o PapilloCheck a vantagem de ser um método que evita a subjetividade da leitura dos genótipos de HPV. .
PURPOSE: The aim of this study was to compare the performance of two human papillomavirus (HPV) genotyping techniques, Linear Array and PapilloCheck, in women with high-grade squamous intraepithelial lesion (HSIL). METHODS: A total of 88 women with cytological diagnosis of HSIL were recruited at 2 reference centers in cervical pathology in Salvador, Bahia, Brazil, from July 2006 to January 2009. After the cytological diagnosis of HSIL, cervix cells were collected to determine the HPV genotype and a biopsy was obtained under colposcopic vision for histopathological analysis. After the confirmation of CIN2+ by histopathology, HPV genotyping was performed on 41 women by the Linear Array and PapilloCheck methods. RESULTS: Both tests showed an overall concordance rate for HPV detection of 97.2% (35/36). Of the 36 valid samples, 35 (97.2%) were positive in both tests and 1 (2.8%) was discordant, with the Linear Array indicating the presence of multiple types. The most prevalent HPV genotypes detected by the Linear Array technique were HPV 16, HPV 56, HPV 35, HPV 45, and HPV 70; and those detected by the PapilloCheck technique were HPV 16, HPV 56, HPV 11, HPV 35, and HPV 42. A similar rate of infection with multiple HPV types was observed with the two tests (72.5% with the Linear Array and 75.0% with the PapilloCheck). CONCLUSIONS: Linear Array genotyping assay and PapilloCheck showed equivalent performance for the detection of oncogenic HPV types in women with HSIL, with PapilloCheck having the advantage of being a method that avoids subjectivity when reading the HPV genotypes. .
Subject(s)
Humans , Autophagy/physiology , Colorectal Neoplasms/etiology , Colorectal Neoplasms/therapy , Disease Progression , PrognosisABSTRACT
Objetivo: Este trabalho descreve um estudo da avaliação do conhecimento do público leigo e de profissionais de saúde sobre a cirurgia e traumatologia buco-maxilo-facial na cidade de Fortaleza, CE. Metodologia: Por meio de questionários, foram entrevistados acadêmicos e profissionais cirurgiões-dentistas, médicos, enfermeiros e o público leigo. Foram descritos casos clínicos e solicitada aos entrevistados a escolha de um especialista para tratar cada caso, dentre eles o cirurgião-plástico, o otorrinolaringologista, o cirurgião buco-maxilo-facial, o cirurgião de cabeça e pescoço e "outros". A análise dos resultados consistiu na construção de tabelas de contingência, com teste do Qui-Quadrado (X²) e associação V de Cramer. Nas comparações, o nível de significância adotado no presente estudo foi de α= 0,05. Resultados: Verificou-se que, para a fratura de nariz, câncer de língua, aumento de volume no pescoço, cirurgia estética do nariz e insatisfação com a estética facial, todos os grupos foram classificados com conhecimento RUIM. Para a remoção de glândula salivar, biópsia de lesões na boca, tumor benigno de mandíbula, aumento de volume na mandíbula, criança com fissura labial, palatal e lábio-palatal, os grupos da medicina foram classificados com conhecimento RUIM. Conclusão: Concluiu-se que o estudo da avaliação do conhecimento sobre a CTBMF revela dados de conhecimento insatisfatório em relação aos grupos estudados.
Purpose: This paper describes a study assessing the knowledge of the lay public and health professionals about the oral and maxillofacial surgery in the city of Fortaleza, CE. Methodology: Through questionnaires, were addressed to academics and professionals of the dentistry, medical, nursing and the lay public. It described several cases of clinical and asked the respondents to choose a specialist to treat each case, including the plastic surgeon, ENT, oral and maxillofacial surgeon, head and neck surgeon and the "other". The analysis consisted in the construction of contingency tables, applying the chi-square (X ²) and association of Cramer V. In comparison, bilateral tests were used where the value of the level of significance adopted in this study was α = 0.05. Results: It was found that for fracture of nose, tongue cancer, increase in volume in the neck, cosmetic surgery of the nose and dissatisfaction with facial aesthetics, that all groups were classified as knowledge BAD. To removal of salivary gland, biopsy of lesions in the mouth, benign tumor of mandible, increase in volume in the mandible, children with cleft lip, palate and lip-palate, groups 3 and 4 were classified with knowledge BAD. Conclusion: It was concluded that the sample of the results of the study assessing the knowledge of the data reveals OMFS of poor knowledge in relation to groups.
ABSTRACT
Human Papilloma Virus (HPV) plays a central role in the development of cervical cancer. However, other coexisting factors, such as HIV infection, must be present for this to occur. We evaluated the prevalence of HPV in HIV-positive and HIV-negative patients in the city of Salvador , Bahia, Brazil, and determined the most prevalent types of HPV in these patients. Fifty-five cases were selected from among patients attending three institutions providing cervical pathology services in the city of Salvador. HIV testing (Elisa/WB), HPV-DNA testing by PCR, colposcopy, cytology and biopsy were carried out in all patients. The histopathological results were classified as follows: 11 cases were normal/negative for neoplasia, 15 were diagnosed as cervical intraepithelial neoplasia grade 1 (CIN 1), 10 were CIN 2, 15 cases were CIN 3 and there were four cases of invasive squamous cell carcinoma. Among the 55 patients studied, 43 tested positive for HPV-DNA and 20 for HIV. All HIV-positive patients were positive for HPV-DNA. The most prevalent types of HPV were HPV 16, 52, 58, 53, 54, 33 and 51, and there was little difference between the groups of HIV-positive and HIV-negative patients with respect to the type of HPV encountered. The HIV-positive patients were found to be infected with a greater number of types of HPV than the HIV-negative patients. This study corroborates the existence of regional variations in the distribution of certain types of HPV, which is probably due to the particular ethnic constitution found in this region of Brazil.